Sequence sensitivity of breathing dynamics in heteropolymer DNA.
نویسندگان
چکیده
We study the fluctuation dynamics of localized denaturation bubbles in heteropolymer DNA with a master equation and complementary stochastic simulation based on novel DNA stability data. A significant dependence of opening probability and waiting time between bubble events on the local DNA sequence is revealed and quantified for a biological sequence of the T7 bacteriophage. Quantitative agreement with data from fluorescence correlation spectroscopy is demonstrated.
منابع مشابه
Breathing dynamics in heteropolymer DNA.
While the statistical mechanical description of DNA has a long tradition, renewed interest in DNA melting from a physics perspective is nourished by measurements of the fluctuation dynamics of local denaturation bubbles by single molecule spectroscopy. The dynamical opening of DNA bubbles (DNA breathing) is supposedly crucial for biological functioning during, for instance, transcription initia...
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ورودعنوان ژورنال:
- Physical review letters
دوره 97 12 شماره
صفحات -
تاریخ انتشار 2006